PCR-based assay for mating type and diploidy in Chlamydomonas

PCR-based assay for mating type and diploidy in Chlamydomonas.

Molecular mating type assay for Fusarium circinatum.

A rapid and reliable mating type assay for Fusarium circinatum was created by applying primers specific for the MAT1-1 and MAT1-2 mating type alleles to genomic DNA in a single PCR. A similar approach may be applied to fungi not previously shown to reproduce sexually, thus enabling studies of population structure and inheritance.

Mating systems and the evolutionary transition between haploidy and diploidy

According to the ‘masking hypothesis’, diploids gain an immediate fitness advantage over haploids because diploids, with two copies of every gene, are better able to survive the effects of deleterious recessive mutations. Masking in diploids is, however, a double-edged sword: it allows mutations to persist over time. In contrast, deleterious mutations are revealed in haploid individuals and are...

Genetic structure of the mating-type locus of Chlamydomonas reinhardtii.

Portions of the cloned mating-type (MT) loci (mt(+) and mt(-)) of Chlamydomonas reinhardtii, defined as the approximately 1-Mb domains of linkage group VI that are under recombinational suppression, were subjected to Northern analysis to elucidate their coding capacity. The four central rearranged segments of the loci were found to contain both housekeeping genes (expressed during several life-...

study on fertility status and determination of frequency of mating type alleles in a and d mating populations of gibberella fujikuroi from rice and corn based on pcr

fertility status and distribution of mating type alleles of gibberella moniliformis and g. intermedia, both the causal agents of the foot rot and bakanae of rice, as well as stalk and root rot of corn were studied using specific primers. one hundred and twenty eight single-spore isolates were collected from rice growing areas of guilan and mazandaran and from various corn-growing areas of iran....